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live/dead fixable blue dead cell stain kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher live/dead fixable blue dead cell stain kit
    Live/Dead Fixable Blue Dead Cell Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+dead+fixable+blue+dead+cell+stain+kit/live+dead+fixable+aqua+dead+cell+stain+kit/pmc12268011-138-9-16
    Average 90 stars, based on 1 article reviews
    live/dead fixable blue dead cell stain kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHCII complexes.
    Article Snippet: .. In brief, cells were stained with surface markers, washed, and resuspended in PBS with fluorescent reactive fixable viability dye (LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for ultraviolet excitation, Invitrogen, catalog no. L34961). ..

    Article Title: Beyond Antagonism: IL-4 Exploits TNF signaling to Shape Its Gene Expression Signature in Monocytes and Macrophages
    Article Snippet: .. Cells were centrifuged to remove digestion buffer and red blood cells were lysed with ACK lysing buffer (Lonza) for 5 min. After washing with FACS buffer (PBS containing 0.5% BSA and 1 mM EDTA), cells were then filtered with 70 μm cell strainer and stained with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific) in PBS. .. After washing with PBS, cells were stained with antibodies including F4/80 (BioLegend, BM8), Ly6G (BioLegend, 1A8), CD11b (BioLegend, M1/70), MHC II (BioLegend, M5/114.15.2), CD206 (BioLegend, C068C2) and CD45 (BioLegend, 30-F11).

    Article Title: Kupffer cell and recruited macrophage heterogeneity orchestrate granuloma maturation and hepatic immunity in visceral leishmaniasis.
    Article Snippet: Red blood cells were lysed using ACK lysis buffer (Lonza), and the remaining cells were washed once with 1x PBS (Gibco). .. All cells were stained using the LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific) at a 1:1000 dilution in 1 × PBS, for 20minutes at 4 °C in the dark. .. Nonspecific labeling was blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (Biolegend) at a 1:100 dilution.

    Article Title: SUMOylation controls Hu antigen R posttranscriptional activity in liver cancer
    Article Snippet: .. Mitochondrial mass was assessed by fluorescent labeling with MitoTracker Green FM probe (M7514, Invitrogen) in combination with LIVE/DEAD fixable blue dead cell stain kit (L23105, Invitrogen), by following the manufacturer’s recommendations. ..

    Article Title: Standard-Dose Intradermal Influenza Vaccine Elicits Cellular Immune Responses Similar to Those of Intramuscular Vaccine in Men With and Those Without HIV Infection
    Article Snippet: After 1 hour of infection, GolgiPlug (1:1000, BD Biosciences) was added, and cells were incubated overnight. .. Cells were then stained with a Live/Dead Fixable Blue Dead Cell Stain Kit (Invitrogen), followed by surface staining with CD4-AmCyan (eBioscience), CD8-BV605, CD56-Alexa Fluor 700, and CD69-APC-Cy7 and, finally, by intracellular staining with interferon γ (IFN-γ)–phycoerythrin-Cy7, tumor necrosis factor α (TNF-α)–phycoerythrin, and interleukin 2 (IL-2)–Pacific blue after fixation and permeabilization with BD Cytofix/Cytoperm (BD Biosciences). .. Samples were acquired on a BD LSRII cytometer and analyzed using FlowJo and Simplified Presentation of Incredibly Complex Evaluations (SPICE) software [ 16 ].

    Article Title: The Triterpenoid CDDO-Methyl Ester Reduces Tumor Burden, Reprograms the Immune Microenvironment, and Protects from Chemotherapy-Induced Toxicity in a Preclinical Mouse Model of Established Lung Cancer
    Article Snippet: Single cells were resuspended in a 1:1 solution of Brilliant Violet buffer (BD Bioscience, Franklin Lakes, NJ, USA):PBA and stained for 30 min on ice using an optimized antibody panel [ ] for immune cell targets including αCD45 (BioLegend, San Diego, CA, USA, BV510 #103138), αCD11b (BioLegend PE/Cyanine7 #101216), αCD11c (BioLegend PE/Cyanine5 #117316), αCD64 (BioLegend BV711 #139311), αCD206 (BioLegend BV421 #141717), αPD-L1 (BioLegend PE-Dazzle594 #124324), αCD4 (Miltenyi Biotrc FITC, Gaithersburg, MD, USA, #130-120-819), αCD8 (Invitrogen BUV805 #368-0081-82), αCD25 (BioLegend PE #101904), αCD24 (BioLegend BV605 #101827), and αCD107a (BioLegend Alexa Fluor 647 #121610). .. Cells were stained for viability with Live/Dead Fixable Blue Dead Cell Stain Kit (Invitrogen #L23105). .. Cells were permeabilized using the BD Biosciences Cytofix/Cytoperm Fixation/Permeabilization kit (#554714) and stained for the intracellular target αFoxP3 (Invitrogen Alexa Fluor 700 #56-5773-80).

    Article Title: Kupffer cell and recruited macrophage heterogeneity orchestrate granuloma maturation and hepatic immunity in visceral leishmaniasis
    Article Snippet: Red blood cells were lysed using ACK lysis buffer (Lonza), and the remaining cells were washed once with 1x PBS (Gibco). .. All cells were stained using the LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific) at a 1:1000 dilution in 1 × PBS, for 20 minutes at 4 °C in the dark. .. Nonspecific labeling was blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (Biolegend) at a 1:100 dilution.

    FACS:

    Article Title: Beyond Antagonism: IL-4 Exploits TNF signaling to Shape Its Gene Expression Signature in Monocytes and Macrophages
    Article Snippet: .. Cells were centrifuged to remove digestion buffer and red blood cells were lysed with ACK lysing buffer (Lonza) for 5 min. After washing with FACS buffer (PBS containing 0.5% BSA and 1 mM EDTA), cells were then filtered with 70 μm cell strainer and stained with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific) in PBS. .. After washing with PBS, cells were stained with antibodies including F4/80 (BioLegend, BM8), Ly6G (BioLegend, 1A8), CD11b (BioLegend, M1/70), MHC II (BioLegend, M5/114.15.2), CD206 (BioLegend, C068C2) and CD45 (BioLegend, 30-F11).

    Labeling:

    Article Title: SUMOylation controls Hu antigen R posttranscriptional activity in liver cancer
    Article Snippet: .. Mitochondrial mass was assessed by fluorescent labeling with MitoTracker Green FM probe (M7514, Invitrogen) in combination with LIVE/DEAD fixable blue dead cell stain kit (L23105, Invitrogen), by following the manufacturer’s recommendations. ..

    other:

    Article Title: CCR2-driven monocyte recruitment is protective against radiotherapy-induced intestinal toxicity
    Article Snippet: PBMC were washed in PBS plus BN, incubated for 30 mins at room temperature with 1:1000 LIVE/DEADTM Fixable Blue Dead Cell Stain Kit (Invitrogen) in PBS plus BN, washed in FACS buffer (PBS, 1% FCS, 50U/ml BN), incubated with Trustain FcX Fc block (1:100 in FACS buffer) for 15 min at 4 °C, washed in FACS buffer and then incubated with 100μl of the antibody staining cocktail in FACS buffer for 30 mins at 4°C.



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